The basal proton conductance of mitochondria depends on adenine nucleotide translocase content Academic Article uri icon

abstract

  • The basal proton conductance of mitochondria causes mild uncoupling and may be an important contributor to metabolic rate. The molecular nature of the proton-conductance pathway is unknown. We show that the proton conductance of muscle mitochondria from mice in which isoform 1 of the adenine nucleotide translocase has been ablated is half that of wild-type controls. Overexpression of the adenine nucleotide translocase encoded by the stress-sensitive B gene in Drosophila mitochondria increases proton conductance, and underexpression decreases it, even when the carrier is fully inhibited using carboxyatractylate. We conclude that half to two-thirds of the basal proton conductance of mitochondria is catalysed by the adenine nucleotide carrier, independently of its ATP/ADP exchange or fatty-acid-dependent proton-leak functions.

authors

  • Brand, Martin D
  • Pakay, Julian L
  • Ocloo, Augustine
  • Kokoszka, Jason
  • Wallace, Douglas C
  • Brookes, Paul S
  • Cornwall, Emma J

publication date

  • December 1, 2005