Lethal giant larvae-1 deficiency enhances the CD8+ effector T-cell response to antigen challenge in vivo Academic Article uri icon

abstract

  • Lethal giant larvae-1 (Lgl-1) is an evolutionary conserved protein that regulates cell polarity in diverse lineages; however, the role of Lgl-1 in the polarity and function of immune cells remains to be elucidated. To assess the role of Lgl-1 in T cells, we generated chimeric mice with a hematopoietic system deficient for Lgl-1. Lgl-1 deficiency did not impair the activation or function of peripheral CD8(+) T cells in response to antigen presentation in vitro, but did skew effector and memory T-cell differentiation. When challenged with antigen-expressing virus or tumor, Lgl-1-deficient mice displayed altered T-cell responses. This manifested in a stronger antiviral and antitumor effector CD8(+) T-cell response, the latter resulting in enhanced control of MC38-OVA tumors. These results reveal a novel role for Lgl-1 in the regulation of virus-specific T-cell responses and antitumor immunity.

authors

  • Ramsbottom, KM
  • Sacirbegovic, F
  • Hawkins, ED
  • Kallies, A
  • Belz, GT
  • Van Ham, V
  • Haynes, NM
  • Durrant, MJ
  • Humbert, PO
  • Russell, SM
  • Oliaro, J

publication date

  • 2016