Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity Academic Article uri icon

abstract

  • The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.

authors

  • Hawkins, ED
  • Oliaro, J
  • Kallies, A
  • Belz, GT
  • Filby, A
  • Hogan, T
  • Haynes, N
  • Ramsbottom, KM
  • Van Ham, V
  • Kinwell, T
  • Seddon, B
  • Davies, D
  • Tarlinton, D
  • Lew, AM
  • Humbert, PO
  • Russell, SM

publication date

  • 2013