Overcoming solvent mismatch limitations in 2D-HPLC with temperature programming of isocratic mobile phases Academic Article uri icon


  • Solvent incompatibility is a limiting factor when implementing 2D-HPLC. A programmed temperature gradient in the first dimension was developed to overcome this limitation by allowing an isocratic separation while maintaining selectivity.


  • Holland, BJ
  • Conlan, XA
  • Francis, PS
  • Barnett, NW
  • Stevenson, PG

publication date

  • 2016