Preparation of B lymphocyte-specific alloantisera by skin implant immunization of cattle Academic Article uri icon

abstract

  • B lymphocyte-specific antisera were prepared by immunizing cattle on either one or two occasions with a subcutaneous implant of allogeneic skin and subsequently absorbing the antisera with platelets. After absorption 15/26 antisera displayed B lymphocyte-specific activity. Titres against B-enriched cells were 8-64 while residual titres against B-depleted cells were 1-8. In comparison, 3-6 immunizations with allogeneic leucocytes produced antisera of similar peak cytotoxic titres against donor PBL, and after platelet absorption 8/15 antisera displayed B lymphocyte-specific activity. Titres against B-enriched cells were 8-64 while residual titres against B-depleted cells were 2-8. The skin implant method was less time-consuming than the leucocyte immunization method.

publication date

  • January 1992