The Survival of Skeletal Muscle Myoblasts in Vitro Is Sensitive to a Donor of Nitric Oxide and Superoxide, SIN-1, but Not to Nitric Oxide or Peroxynitrite Alone Academic Article uri icon

abstract

  • The survival of skeletal muscle myoblasts in culture after exposure either to a donor of NO, sodium nitroprusside (SNP), or ethanamine, 2,2'-(hydroxynitrosohydrazono)bis-(DETA NONOate), or to a donor of both NO and O(-)(2), 3-morpholinosydnonimine hydrochloride (SIN-1), was investigated. SIN-1 reduced clonogenic survival markedly but donors of NO alone did not. The injurious effect of SIN-1 was prevented by oxyhemoglobin or by uric acid but not by superoxide dismutase. The exposure of myoblasts to authentic peroxynitrite (ONOO(-)) or to DETA NONOate in the presence of an O(-)(2)-generating system did not reduce their survival. The results show that NO or ONOO(-) alone is not detrimental to myoblast survival and suggest that SIN-1 toxicity is, at least in part, mediated by H(2)O(2) in this myoblast culture system.

authors

  • Lepore, DA
  • Stewart, AG
  • Tomasi, A
  • Anderson, RL
  • Hurley, JV
  • Morrison, WA

publication date

  • August 1999