OBJECTIVES:To investigate the acute glyacaemic response to accumulated or single bout walking exercise in apparently healthy adults. DESIGN:Three arm, randomised crossover control study. METHODS:Ten adults (age: 50±12.6 y; BMI 29.0±5.4kgm-2) completed three separate trials comprising three 10-min walking bouts after breakfast, lunch, and dinner (APPW), a single 30-min walking bout after dinner only (CPPW), or a no-exercise control (NOEX). Participants walked on a treadmill at a moderate intensity of 55%-70% heart rate reserve. Two-hour postprandial glucose response was assessed using a continuous glucose monitor. RESULTS:There was a difference in the pattern of the glucose response between the trials during the two hours following dinner (p<0.001). Postprandial dinner glucose concentrations were not different between APPW and CPPW but were up to 1.01mmolL-1 lower than NOEX (partial eta2=0.21, p=0.041). CONCLUSIONS:Ten minutes of moderate intensity walking completed 30min after each meal lowers postprandial dinner glucose concentrations in comparison to no-exercise, and reduces glucose by a similar magnitude as a single 30-min bout after the evening meal. Short bouts of exercise after each meal may be recommended to minimise glucose elevations after dinner that might increase risk of cardiometabolic disease.