OBJECTIVE:No studies have objectively measured habitual usage of sit-stand workstations. METHODS:Eighteen full-time office workers participated (47.9 ± 9.2 years, 61% female). Sitting time was objectively measured (activPAL, 24 h/7 days), and time at desk, desk position, and perceptions of desk use were self-reported. RESULTS:Participants sat for 39% of their daily workstation time, and changed workstation position twice daily. The most common reasons for standing included back pain (44%) and tiredness (22%). The majority of participants received no workstation occupational health (72%) or educational (61%) information. Workstation standing time had a significant moderate correlation with total daily standing time (P = 0.02). CONCLUSION:Office workers with sit-stand workstations rarely change desk position, and there is no relationship between the time spent sitting at the workstation, and total daily sitting time. Education about the workstations was limited.